Construction of an Alternative NAD <sup>+</sup> De Novo Biosynthesis Pathway
نویسندگان
چکیده
Nicotinamide adenine dinucleotide (NAD+) is a life essential molecule involved in versatile biological processes. To date, only two de novo biosynthetic routes to NAD+ are described, both of which start from proteinogenic amino acid and tightly controlled. Here, quinolinic pathway starting chorismate, provides an alternative route (named as the C3N pathway) biosynthesis, established. Significantly, yields extremely high cellular concentrations NAD(H) E. coli. Its utility cofactor engineering demonstrated by introducing four-gene module cell factories achieve higher production 2,5-dimethylpyrazine develop efficient C3N-based whole-cell bioconversion system for preparing chiral amines. The wide distribution abundance chorismate most kingdoms implies general modulating levels organisms.
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ژورنال
عنوان ژورنال: Advanced Science
سال: 2021
ISSN: ['2198-3844']
DOI: https://doi.org/10.1002/advs.202004632